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RAS BiologyМолекулярная биология Molecular Biology

  • ISSN (Print) 0026-8984
  • ISSN (Online) 3034-5553

Coordinated Expression and Methylation of microRNAs: Role in Common Biological Processes and Signaling Pathways in Breast Cancer

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PII
S30345553S0026898425050075-1
DOI
10.7868/S3034555325050075
Publication type
Article
Status
Published
Authors
E.A. Filippova
  • Affiliation: Institute of General Pathology and Pathophysiology
I.V. Pronina
  • Affiliation: N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences
V.I. Loginov
  • Affiliation: Institute of General Pathology and Pathophysiology
T.P. Kazubskaya
  • Affiliation: N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation
E.A. Braga
  • Affiliation: Institute of General Pathology and Pathophysiology
Volume/ Edition
Volume 59 / Issue number 5
Pages
821-834
Abstract
Breast cancer remains one of the leading causes of cancer mortality among women, and the study of epigenetic mechanisms is an important task of molecular oncology in breast cancer. In this study, we analyzed the expression levels of 8 microRNAs (miR-125b-5p, -127-5p, -129-5p, -132-3p, -148a-3p, -193a-5p, -24-2-5p, -34b-3p) and methylation of promoter regions of 7 microRNA genes in a representative sample of 40 and 70 paired samples of tumor and normal breast tissue, respectively, and showed hypermethylation of promoter regions of 7 genes and statistically significant decrease in expression levels of 8 microRNAs in tumor. For three genes (MIR125B-1, MIR129-2, MIR148A), inverse relationships between methylation and expression (r < −0.5) were revealed, indicating their possible epigenetic regulation. Statistically significant positive correlations of expression levels were revealed for 7 pairwise combinations of miRNAs, suggesting their coordinated functioning. Indeed, for the pairs miR-127-5p/miR-125b-5p, miR-148a-3p/miR-125b-5p, miR-148a-3p/miR-132-3p, miR-34b-3p/miR-193a-5p, common mRNA targets and involvement in biological processes, including pathways associated with epigenetic regulation, proliferation and metastasis, were revealed. The miRNA–mRNA regulatory network constructed involving DNMTs and EZH2 highlights their potential role in breast cancer progression and demonstrates diagnostic and prognostic significance.
Keywords
микроРНК коэкспрессия гиперметилирование рак молочной железы анализ обогащения GO KEGG
Date of publication
31.01.2026
Year of publication
2026
Number of purchasers
0
Views
65

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