RAS BiologyМолекулярная биология Molecular Biology

  • ISSN (Print) 0026-8984
  • ISSN (Online) 3034-5553

ASSEMBLY OF A COMPLEX OF IMMATURE 30S RIBOSOMAL SUBUNIT AND Era GTPase MATURATION FACTOR FROM STAPHYLOCOCCUS AUREUS IN VITRO FOR STRUCTURAL STUDIES

PII
S30345553S0026898425040068-1
DOI
10.7868/S3034555325040068
Publication type
Article
Status
Published
Authors
Volume/ Edition
Volume 59 / Issue number 4
Pages
599-606
Abstract
Staphylococcus aureus Era GTPase is one of the ribosome maturation factors. Era GTPase is involved in the late stages of small (30S) ribosome subunit assembly and regulates formation of the entire 70S ribosome. Study of the structure of the Era and 30S subunit complex from S. aureus will help in studying the processes of ribosome maturation and mechanisms of protein synthesis regulation in this pathogenic microorganism. This paper presents protocols for obtaining Era GTPase and immature 30S ribosome subunits from S. aureus, as well as a protocol for assembling their complex for further structural studies.
Keywords
Staphylococcus aureus Era GTPаза фактор созревания рибосомы рибосома криоэлектронная микроскопия сборка 30S субъединицы рибосомы
Date of publication
31.01.2026
Year of publication
2026
Number of purchasers
0
Views
68

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